The control of enzyme reactions is of extraordinary importance for diverse areas in fundamental and applied research such as (nano)biotechnology, synthetic biology, and biomedicine. In the context of DNA origami, enzyme reactions are of particular relevance for the structural integrity of the DNA nanostructures in adverse environments. In physiological media, for instance, nucleases digest DNA origami nanostructures and thereby limit their in-vivo life and circulation times. Ligases, on the other hand, can be used to seal discontinuities in the DNA backbone, so-called nicks, and thereby increase the thermal stability of DNA origami nanostructures. Both effects are critically influenced by DNA origami superstructure, both on a local and a global level. We thus investigate the effects of various structural and design parameters on ligase and nuclease reactions in different DNA origami nanostructures with the aim to develop general design rules for controlling enzyme reactions in these nanostructures.
- Enhancing the stability of DNA origami nanostructures: staple strand redesign versus enzymatic ligation, S. Ramakrishnan, L. Schärfen, K. Hunold, S. Fricke, G. Grundmeier, M. Schlierf, A. Keller, and G. Krainer, Nanoscale 11, 16270 (2019)
- Real‐Time Observation of Superstructure‐Dependent DNA Origami Digestion by DNase I Using High‐Speed Atomic Force Microscopy, S. Ramakrishnan, B. Shen, M. A. Kostiainen, G. Grundmeier, A. Keller, and V. Linko, ChemBioChem 20, 2818 (2019) (Cover Picture)